Journal: PLOS ONE

Article Title: Low-frequency whole-body vibration can enhance cartilage degradation with slight changes in subchondral bone in mice with knee osteoarthritis and does not have any morphologic effect on normal joints

doi: 10.1371/journal.pone.0270074

Figure Lengend Snippet: Oligonucleotide primers for RT-PCR.

Article Snippet: Immunohistochemistry staining for Collagen II (Col2a1) (1:100, bs-10589R; Bioss, Beijing, China), Aggrecan (Acan) (1:200, bs-11655R; Bioss), MMP3(1:50, Ab52915; Abcam, Cambridge, UK), MMP13 (1:200, Ab39012; Abcam), IL6 (1:100, ab290735; Abcam) and TNFα(1:1000, ab307164; Abcam) were conducted on paraffin sections following appropriate antigen retrieval methods.

Techniques:

Journal: PLOS ONE

Article Title: Low-frequency whole-body vibration can enhance cartilage degradation with slight changes in subchondral bone in mice with knee osteoarthritis and does not have any morphologic effect on normal joints

doi: 10.1371/journal.pone.0270074

Figure Lengend Snippet: Compared to the baseline group with sham DMM, the group that underwent DMM surgery exhibited an obvious increase in Acan, Col2a1, MMP3 and MMP13. The mouse model of OA eight weeks after surgery showed a further increase in MMP3 and MMP13, while Acan and Col2a1 showed a decrease, compared with those of the baseline OA group. Obvious increases in TNFα and IL6 were also presented in the mouse model of eight weeks after surgery compared to those in the matched group four weeks after DMM surgery. All the genes detected had a significant decrease in the group with OA and WBV compared with the non-vibrated group. (n = 6). Data are expressed as the mean ± SD.** P <0.01.

Article Snippet: Immunohistochemistry staining for Collagen II (Col2a1) (1:100, bs-10589R; Bioss, Beijing, China), Aggrecan (Acan) (1:200, bs-11655R; Bioss), MMP3(1:50, Ab52915; Abcam, Cambridge, UK), MMP13 (1:200, Ab39012; Abcam), IL6 (1:100, ab290735; Abcam) and TNFα(1:1000, ab307164; Abcam) were conducted on paraffin sections following appropriate antigen retrieval methods.

Techniques:

Journal: PLOS ONE

Article Title: Low-frequency whole-body vibration can enhance cartilage degradation with slight changes in subchondral bone in mice with knee osteoarthritis and does not have any morphologic effect on normal joints

doi: 10.1371/journal.pone.0270074

Figure Lengend Snippet: Compared to the baseline group with sham DMM, the group that underwent DMM surgery exhibited an obvious decrease in Acan and Col2a1, and increase in MMP3, MMP13, IL6 and TNFα. The mouse model of OA eight weeks after surgery showed a further increase in MMP3, MMP13, and an obivious decrease in Acan, Col2a1, compared with those of the baseline OA group. All the proteins detected had a significant decrease in the group DMM+WBV compared with the DMM+NON-WBV group. (n = 6).

Article Snippet: Immunohistochemistry staining for Collagen II (Col2a1) (1:100, bs-10589R; Bioss, Beijing, China), Aggrecan (Acan) (1:200, bs-11655R; Bioss), MMP3(1:50, Ab52915; Abcam, Cambridge, UK), MMP13 (1:200, Ab39012; Abcam), IL6 (1:100, ab290735; Abcam) and TNFα(1:1000, ab307164; Abcam) were conducted on paraffin sections following appropriate antigen retrieval methods.

Techniques:

Journal: Inflammation

Article Title: Loss of the miR-221/222 Cluster Promotes the Pathogenesis of Osteoarthritis in Inflamed Mouse Chondrocytes and Osteoarthritis Models

doi: 10.1007/s10753-025-02411-4

Figure Lengend Snippet: Downregulation of miR-221 and miR-222 expression in OA tissue and IL-1β-stimulated chondrocytes. ( A-B ) Safranin O staining of the knee joints of OA model mice post-DMM surgery at the indicated time point showed progressive deterioration of articular cartilage. ( C ) Decreased expression of miR-221 and miR-222 in cartilage tissues from mouse joints ( n = 5 biologically independent samples). ( D ) Validation of in vitro OA model induction: NO levels were measured in the supernatant of IL-1β (10 ng/ml)-stimulated chondrocytes, along with mRNA expression levels of MMP-13 and COL2A1 in the same cells ( n = 5 biologically independent samples). ( E-F ) Time- and dose-dependent reduction in miR-221 and miR-222 expression in chondrocytes treated with IL-1β ( n = 5 independent biological samples). Data are presented as mean ± SEM. p values were calculated using one-way ANOVA followed by Tukey’s post hoc test

Article Snippet: After that, cells were incubated with anti-MMP-13 or COL2A1 antibodies (Both 1:500, Bioss), followed by FITC-conjugated goat anti-rabbit IgG (1:500, Proteintech).

Techniques: Expressing, Staining, Biomarker Discovery, In Vitro

Journal: Inflammation

Article Title: Loss of the miR-221/222 Cluster Promotes the Pathogenesis of Osteoarthritis in Inflamed Mouse Chondrocytes and Osteoarthritis Models

doi: 10.1007/s10753-025-02411-4

Figure Lengend Snippet: Exacerbation of OA progression in miR-221/222 cluster KO mice. ( A ) X-ray images of mouse joints 8 weeks post-DMM showing increased osteophytes, irregular joints, and pronounced joint stenosis in the KO + DMM group compared with the WT + DMM group. ( B-F ) Histological staining of joint sections at 8 weeks post-DMM confirms more severe degradation of cartilage and synovial fibrosis in the KO + DMM group ( n = 5 independent biological samples). ( G-K ) Western blot analysis indicates elevated type I collagen expression ( G ), while immunofluorescence analysis shows reduced COL2A1 levels ( H , I ) and increased MMP-13 expression ( J , K ) in KO + DMM joints at 8 weeks post-surgery ( n = 3 independent biological samples). For all the graphs, the values represent the mean ± SEM. p values were calculated via one-way ANOVA, followed by Tukey’s post hoc test

Article Snippet: After that, cells were incubated with anti-MMP-13 or COL2A1 antibodies (Both 1:500, Bioss), followed by FITC-conjugated goat anti-rabbit IgG (1:500, Proteintech).

Techniques: Staining, Western Blot, Expressing, Immunofluorescence

Journal: Inflammation

Article Title: Loss of the miR-221/222 Cluster Promotes the Pathogenesis of Osteoarthritis in Inflamed Mouse Chondrocytes and Osteoarthritis Models

doi: 10.1007/s10753-025-02411-4

Figure Lengend Snippet: Increased inflammatory mediator levels and the expression of chondrocyte OA-related factors in miR-221/222 cluster KO mice. ( A-C ) Levels of IL-6 ( A ), TNF-α ( B ), and NO ( C ) production in IL-1β (10 ng/ml) treated chondrocyte-free supernatant from WT and miR-221/222 cluster KO mice ( n = 5 independent biological samples). ( D-E ) Expression of inflammation-related proteins (COX2 and iNOS), OA-associated proteins (MMP-13), and COL2A1 in IL-1β (10 ng/ml) treated chondrocytes from WT and miR-221/222 cluster KO mice ( n = 3 independent biological samples). ( F-I ) Immunofluorescence confirming COL2A1 ( F and G ) and MMP-13 ( H and I ) expression in IL-1β (10 ng/ml) treated chondrocytes from WT and miR-221/222 cluster KO mice ( n = 5 independent biological samples). For all the graphs, the values represent the mean ± SEM. p values were calculated via one-way ANOVA, followed by Tukey’s post hoc test

Article Snippet: After that, cells were incubated with anti-MMP-13 or COL2A1 antibodies (Both 1:500, Bioss), followed by FITC-conjugated goat anti-rabbit IgG (1:500, Proteintech).

Techniques: Expressing, Immunofluorescence

Journal: Inflammation

Article Title: Loss of the miR-221/222 Cluster Promotes the Pathogenesis of Osteoarthritis in Inflamed Mouse Chondrocytes and Osteoarthritis Models

doi: 10.1007/s10753-025-02411-4

Figure Lengend Snippet: Alleviation of OA progression by the administration of agomir miR-221/222. ( A ) Schematic of the experimental design. ( B–D ) Intra-articular injection of the miR-221/222 agomir for 8 weeks attenuated OA progression in DMM-induced WT mice, as evidenced by improved histological staining ( B ) and decreased MMP-13 expression in joint tissues ( C , D ). E–F) Treatment also enhanced COL2A1 expression levels in the joints. ( n = 9 biologically independent samples). Data are presented as mean ± SEM. p values were determined using one-way ANOVA followed by Tukey’s post hoc test

Article Snippet: After that, cells were incubated with anti-MMP-13 or COL2A1 antibodies (Both 1:500, Bioss), followed by FITC-conjugated goat anti-rabbit IgG (1:500, Proteintech).

Techniques: Injection, Staining, Expressing